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G4103-5ML Cell Counting Kit-8 CCK-8 for Cell Proliferation 5ML in Bottle

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G4103-5ML Cell Counting Kit-8 CCK-8 for Cell Proliferation 5ML in Bottle

Cell Counting Kit-8, referred to as a CCK-8 (or CCK8) kit, is a rapid use of cell proliferation and cytotoxicity, high sensitivity test kit.

The detection principle is that in the case where the electron coupling reagent is present, the WST-8 compound in the detecting agent is reduced by the living cell dehydrogenase to have a water-soluble orange-yellow tetra compound, which is the maximum absorption at 450 nm. peak.
The amount of formazan and the live cells is proportional, the more the cell proliferation, the deeper the color; the larger the cell toxicity, the color is even. For the same cells, the depth of the color and the number of cells are linear. In this kit, WST-8 has no obvious toxicity, which can be used for exogenous cytokines such as cell proliferation detection, and drug-induced cytotoxicity detection of cellular toxic reagents, or some drug-induced cell growth inhibition, Is an upgraded version of the MTT detection method.

This kit is convenient, no need to formulate or dilute, no need to collect or wash cells, no need to dissolve the formazan compound, and apply it to the wall and suspension cells. And phenol red and serum have no significant effect on the assay of this rejection.

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