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G4101-200T / 1000T MTT detection kit 1000T Cell viability detection reagent

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G4101-200T / 1000T MTT detection kit 1000T Cell viability detection reagent

This product MTT detection kit is widely used in cell proliferation and activity determination, as well as the detection of drug-to-cytotoxicity. The full name of MTT is 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, and the Chinese name is thiazole blue. The detection principle is that the succinate dehydrogenase in the mitochondria of living cells can reduce the exogenous substrate MTT to water-insoluble blue-purple formazan (Formazan) and deposit in the cells, while dead cells have no such function. Then use dimethyl sulfoxide (DMSO) to dissolve the formazan deposits in the cells, and measure the light absorption value at 490 nm or 570 nm with a microplate reader. Within a certain range of cell numbers, the amount of formazan formation is The number of living cells is directly proportional. According to the measured absorbance value (OD value), the number of living cells can be judged. The larger the OD value, the more the number of living cells and the stronger the cell activity (if the drug toxicity is measured, it means that the drug toxicity is less). When using this kit to detect cell viability, the supernatant needs to be aspirated after the reaction, so it cannot be used for the detection of suspended cells. If you need to detect suspended cells, it is recommended to use other products of our company (G4103 CCK-8 kit).

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