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G3337-50 / 100 SweScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Remover)

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G3337-50 / 100 SweScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Remover)

This product contains 5×Ready-to-use master mix (5×SweScript All-in-One SuperMix for qPCR) and gDNA remover reagents, can remove genomic remnants of the template while the reverse transcription reaction is performed.The kit contained 5×SweScript All-in-One No-RT Control SuperMix as a reverse-transcriptase-free control to determine whether the qPCR template was derived from cDNA.The master mix contains all reagents required for the reverse transcription reaction(SweScript RT, RNase Inhibitor, Random Primer, Oligo(dT)18 Primer, dNTPs and the optimized buffer system). SweScript RT is a mutant reverse transcriptase obtained by in vitro evolutionary screening and is characterized by high thermal stability and processivity. gDNA Remover contains a thermosensitive DNase that is 30 times more active than DNase I and acts only on dsDNA and is susceptible to inactivation under high temperature conditions.

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