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G2081-1L SWE Fast High-resolution Electrophoresis Buffer Powder

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G2081-1L SWE Fast High-resolution Electrophoresis Buffer Powder

Specifiction: Dissolved in 1L water.

Application: Fast and efficient protein separation.

Transportation and Storage: Transport at room temperature, store at dry condition and valid for 24 months.

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The SWE rapid high resolution runing bufferr (powder), optimized from Tris-glycine running buffer, is suitable for use with Tris-HCl SDS-PAGE gel electrophoresis. Compared with the traditional Tris-glycine running buffer, when using this product for electrophoresis, the holding voltage of 200-250 V, about 30 min to complete electrophoresis, was significantly time saving. It also performed better on small molecule protein separation, being able to separate the 10 kDa from the 15 kDa band in prestained protein marker and clearly visible (gel concentration above 8%), to achieve rapid and efficient isolation of proteins.

Technical Specification

Storage

Storage conditions: Room temperature.

Shipping conditions: Room temperature.

Shelf life: 24 months from date of manufacture.

 

Assay Protocol / Procedures

  1. Dissolve in 1000 ml of pure water per bag of running buffer (powder) and use after sufficient dissolution.
  2. Different protein gel concentrations were chosen for different protein molecular weights,  refer to the table below:

    SDS-PAGE Separation gel concentration

    Optimum separation range (kDa)

    (SWE rapid high resolution runing buffer)

    6%

    15-300

    8%

    10-250

    10%

    5-150

    12%

    3-100

    15%

    <60
  3. Constant voltage electrophoresis, 200-250 V, Refer to the following table for electrophoresis time:

Comparison table of electrophoresis voltage and electrophoresis time

Voltage(V)

Estimated electrophoresis time(min)

200

35

220

30

250

25

 

 

Note:

  1. This product is suitable for Tris-HCl system SDS-PAGE gel, for example, our products  G2003?G2037, also applicable to Colorful Acrylamide Kit G2041,G2042,G2043,G2044,G2045,G2060,G2061,G2062, G2063,G2064.
  2. High voltage electrophoresis is easy to produce high heat. Please select the appropriate voltage electrophoresis according to the ambient temperature.
  3. The running solution can be reused for 2-3 times, and it is not recommended to reuse more times.
  4. Please wear lab coat and disposable gloves during operation.

 

For research only, not for clinical diagnosis!

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