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G2075-1ML 5 X SDS-Page Protein Loading Buffer (odorless, reduced type)

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G2075-1ML 5 X SDS-Page Protein Loading Buffer (odorless, reduced type)

5×SDS-PAGE Protein Loading Buffer(Odorless, Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins. The product contains bromophenol blue, which can confirm the progress of electrophoresis.

In addition, the product contains odorless reducing agent instead of dithiothreitol (DTT) or mercaptoethanol, making the protein loading operation safer and healthier, and the water solubility is more stable.

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Package Contents:

Item code Description Qty
G2075-1ML 5×SDS-PAGE Protein Loading Buffer (Odorless,Reducing) 1x1mL
G2075-100ML 5×SDS-PAGE Protein Loading Buffer (Odorless,Reducing) 1x100mL
  Manual 1pc

Technical Specification

Storage and Handling Conditions

Storage at -20°C;Valid for 12 months.

Assay Protocol / Procedures

  1. Allow the tube to reach room temperature and thoroughly mix before use;
  2. Add it to the protein sample to make its working concentration 1×;
  3. Fully denature the protein by heating in 95ºC water bath or metal bath for 10 min;
  4. The samples can be loaded directly after reaching to room temperature;
  5. Usually, electrophoresis can be stopped until the blue dye reaches the bottom of the gel.

Notes

  1. The product must be completely dissolved before use, and stored at -20ºC as soon as possible after use.
  2. Avoid direct contact between reagents and skin.
  3. Please wear lab coat and disposable gloves during operation.
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