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G2015-250ML / 500ML Red Blood Cell Lysis Buffer solution ACK Lysis reagent

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G2015-250ML / 500ML Red Blood Cell Lysis Buffer solution ACK Lysis reagent

Red Blood Cell Lysis Buffer, or ACK Lysis Buffer, is a classic lysis solution used to remove red blood cells, which can fully remove red blood cells without damaging nucleated cells. The tissue cells obtained by lysis of red blood cell lysate do not contain red blood cells, and can be further used for primary culture, cell fusion, flow cytometry analysis, separation and extraction of nucleic acid and protein, etc.

The basic principle is to lyse red blood cells using the principle of cell membrane rupture caused by the difference in intracellular osmotic pressure. The main ingredients include ammonium chloride, potassium bicarbonate, and EDTA-Na2.

Because ammonium ions cannot pass through the cell membrane, but other ions can pass, resulting in a difference in ion concentration inside and outside the cell, the external water diffuses into the cell, causing the red blood cells to expand and achieve the lysis effect.

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Product Variation:

Item code Description Volume
G1505-250ML Red Blood Cell Lysis Buffer 250mL
G1505-500ML 50mL

Technical Specification

Storage condition and transportation

Stored at room temperature, valid for 12 months.

 

Usage Tips:

Tissue cell sample:

  1. Fresh tissues are digested by trypsin or collagenase to form a single cell suspension, centrifuge to discard the supernatant;
  2. Add red blood cell lysate to the cell pellet in a ratio of 1:3-5, gently pipette to mix, and lyse for 1-2 min;
  3. Centrifuge at 800-1000 rpm for 5-8 minutes, and discard the upper red clear liquid by centrifugation;
  4. Collect the precipitated part, add Hank’s solution or serum-free culture solution to centrifuge and wash 2-3 times;
  5. If the lysis is not complete, repeat steps 2 and 3;
  6. Resuspend the cells in an appropriate solution according to the needs of the experiment for subsequent experiments; such as extracting RNA, it is best to use a solution prepared with DEPC water at the beginning of step 4.

 

Blood cells:

  1. Fresh anticoagulant blood, centrifuge and discard the supernatant;
  2. Add red blood cell lysate to the cell pellet in a ratio of 1:6-10, gently pipette to mix, and lyse for 1-5 min;
  3. Centrifuge at 800-1000 rpm for 5-8 minutes, and discard the upper red clear liquid by centrifugation;
  4. Collect the precipitated part, add Hank’s solution or serum-free culture solution to centrifuge and wash 2-3 times;
  5. If the lysis is not complete, repeat steps 2 and 3;
  6. Resuspend the cells with an appropriate solution according to the experimental needs for subsequent experiments; such as extracting RNA, it is best to use a solution prepared with DEPC water from step 4.

 

Precautions

  1. This product is filtered and sterilized. Please pay attention to aseptic operation when using it to avoid bacterial contamination.
  2. If the follow-up test is used for cell culture, it should be done in an ultra-clean workbench during the operation, and attention should be paid to aseptic operation to prevent cells from being contaminated and affecting cell culture.
  3. After centrifugation and washing, if a very small amount of red blood cells are found, the test can be continued without affecting subsequent testing.
  4. Please wear lab coat and disposable gloves during operation.
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