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G2008-1ML 1ml PMSF Phenylmethanesulfonyl Fluoride Protease Inhibitor

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G2008-1ML 1ml PMSF Phenylmethanesulfonyl Fluoride Protease Inhibitor

The synthesis and decomposition of proteins in cells are in dynamic balance under stable conditions.However, when proteins are extracted from cells and tissues in vitro, cells contain many endogenous proteases that can degrade proteins, such as phosphatase and serine protease.These ubiquitous endogenous proteases cause the target protein to break down, thereby affecting the experimental results.

To avoid protein degradation, protease inhibitors are usually added to protein extraction. PMSF (Phenylmethanesulfonyl fluoride), with molecular formula C7H7FO2S and molecular weight 174.19, inhibits serine proteases (such as trypsin, chymotrypsin, thrombin) and mercapto proteases (such as papain) in an irreversible way.

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