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G1514-50T / 100T Annexin V-IF647/PI Cell Apoptosis Detection Kit

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G1514-50T / 100T Annexin V-IF647/PI Cell Apoptosis Detection Kit

Apoptosis is a normal physiological process that occurs in the process of embryonic development and maintenance of tissue homeostasis. It is accompanied by many morphological changes. Among them, the loss of cell membrane is one of the early characteristics of apoptosis. In normal cells, phosphotidylserine (PS) is only distributed on the inner side of the phospholipid bilayer of the cell membrane, but in the early stage of apoptosis, PS will flip from the inner side of the lipid membrane to the outer side, exposing it to the outside of the cell. Annexin V (Annexin V) is a Ca2+-dependent phospholipid-binding protein with high affinity for PS and specifically binds to cells exposed to PS. Therefore, Annexin V is used as one of the indicators for detecting early cell apoptosis. Propidium Iodide (PI) is a nucleic acid dye. It cannot penetrate normal cells with intact cell membranes and early apoptotic cells, but can penetrate the cell membranes of late apoptotic and necrotic cells and stain the nucleus.

 

This product uses the IF647 fluorescent dye molecule labeled Annexin V as a detection probe to detect early cell apoptosis. At the same time, PI is used to distinguish surviving cells from necrotic and late apoptotic cells. Combined use of Annexin V-IF647 and PI, live cells show negative staining (Annexin V-/PI-), early apoptotic cells show single fluorescence positive (Annexin V+/PI-), and late apoptotic cells and necrotic cells show dual fluorescence Positive (Annexin V+/PI+). This kit is suitable for flow cytometry or fluorescence microscope detection.

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