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G1214-100UL Goat anti-mouse secondary antibody For IHC Western blot
The goat anti-mouse secondary antibody of this product is a molecule formed by the combination of horseradish peroxidase (HRP) and goat anti-mouse IgG. It is imported aliquot and can be used for IHC, Western blot and other experiments. This product is suitable for mouse-derived primary antibodies. In IHC and Western blot experiments, HRP-labeled goat anti-rabbit secondary antibodies are combined with rabbit-derived (reacted with antigen first), and then reacted with DAB. DAB is a substrate of horseradish peroxidase (HRP). Under the catalysis of HRP, DAB produces brown precipitates to achieve signal amplification and color development.
Quantity
Technical Specification
Storage conditions:
Wet ice transportation ; store the secondary antibody at -20°C, valid for 12 months.
Instructions for use
For Western or IHC experiments, please refer to the relevant experimental procedures.
For IHC detection, the recommended dilution ratio is 1:200;
For WB detection, the recommended dilution ratio is 1:5000.
Adjust according to the actual color rendering situation.
Precautions
Please wear laboratory clothes and disposable gloves during operation.
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- Protein Extraction
- Gel Preparation
- Protein Electrophoresis
- Protein Transfer
- Antibody
- ECL Kit
- Cell Separation and Digestion
- Nucleus Fluorescence Detection
- Cell Apoptosis Detection
- Cell Proliferation Detection
- Pathological
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- Antigen-Retrieval Solution
- Immunohistochemistry Kit
- Immunofluorescence Staining Kit
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