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G1206-250ML 20x Tris-EDTA Repair Buffer Solution(pH 8.0) Antigen Retrieval Buffer

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G1206-250ML 20x Tris-EDTA Repair Buffer Solution(pH 8.0) Antigen Retrieval Buffer

Immobilization of cells or tissues with paraformaldehyde, formaldehyde or other aldehyde reagents results in cross-linking of proteins, obscuring the antigenic site of the sample, leading to diminished staining signals during immunostaining and even false positive staining results.The protein crosslinking caused by aldehydes is reversible. Under certain conditions, such as high temperature or protease hydrolysis, the original conformation of the protein can be restored. This process is antigen retrieval. Antigen repair is a key step in the process of immunohistochemistry. There are many kinds of antigen repair methods. Different methods can be selected according to the characteristics of tissue types. Some are suitable for thermal repair and some are suitable for enzymatic repair. There are also many different choices to pH value of antigen repair solution due to the characteristics of antigen. The company provides a variety of antigen repair solutions with different pH values, which can meet the needs of a variety of antigen repair.

This product 20×Tris-EDTA repair solution (pH 8.0) is a concentrated repair solution. The main component of the product is 20 mM EDTA. After 20 times dilution, a solution contained 1.0 mM EDTA with pH 8.0 can be obtained.

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