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G1201-1L Citric Acid Buffer Solution Antigen Retrieval Buffer Dry Powder

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G1201-1L Citric Acid Buffer Solution Antigen Retrieval Buffer Dry Powder

After the cells or tissues are fixed with paraformaldehyde, formaldehyde or other aldehyde reagents, they will lead to cross-linking between proteins, thus obscuring the antigen sites of the samples, leading to the weakening of staining signals during immunostaining, and even some false positive staining results. A widely used citric acid buffer (pH6.0) is used in the antigen repair solution, which can effectively remove the cross-linking between proteins caused by aldehyde fixation reagents and fully expose the antigen epitopes in samples such as paraffin sections. Thus, the immunostaining effect is greatly improved.

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Technical Specification

Storage condition:
Stored at room temperature, valid for 12 months.


Usage:
Need to be fully dissolved with 1 L of pure water.
Paraffin sections were placed in 65ºC oven to bake 2h, dewaxed to water, washed three times with PBS, 5 min each time
The slice is placed in 1x citric acid repair buffer solution and put into the microwave to repair 8 min, the repair intensity and time can also be adjusted according to the requirements of the specimen, so as to ensure that the repair solution is between 90-100ºC and avoid boiling and excessive water evaporation. After natural cooling, follow-up operation can be carried out.
 

Notes:

  1. Please use it as soon as possible after dissolution, long-term storage is not recommended.
  2. Please wear lab coat and disposable gloves during operation.
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