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G1005-100ML / 500ML Hematoxylin and Eosin Joint Staining HE Stain Solution

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G1005-100ML / 500ML Hematoxylin and Eosin Joint Staining HE Stain Solution

The acidic nuclei in cells shows high affinity with basic dye (hematoxylin), while the cytoplasm containing alkali substances which show high affinity with acid dye (eosin). When stained with Hematoxylin-Eosin Solution, the nuclei of cells or tissue section shows sharp blue, and the cytoplasm, collagen and muscle fibers show the color of pink to red in different extent. 

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Product Variation:

Item code Description G1005-100ML G1005-500ML
G1005-1 Hematoxylin Stain 100mL 500mL
G1005-2 Eosin Y (alcohol) 100mL 500mL

Technical Specification

Usage:

  1. Deparaffinize sections, 2 changes of xylene, 20 minutes each. Dehydrate in 2 changes of absolute alcohol, 5 minutes each. Rinse 75% alcohol for 5 minutes. Wash briefly in tap water.
  2. Stain in hematoxylin solution for 3-5 minutes. Differentiate in 1% acid alcohol for 30 seconds. Then Bluing in 0.2% ammonia water, wash in running tap water for 5 minutes.
  3. Dehydrate through 85% alcohol and 95% alcohol respectively. Counterstain in Eosin Y solution (alcohol) for 5 minutes.
  4. Dehydrate in 3 changes of absolute alcohol, 5 minutes each. Clear in 2 changes of xylene, 5 minutes each. Mount with resin mounting medium.

Microscopy examination and analysis.

 

Staining result:

Nuclei should be blue, cytoplasm pink to red.

 

Notes:

  1. Low temperature is not good for hematoxylin coloring. You should extend the staining time under low indoor temperature.
  2. The time for deparaffinize should be adjust according to room temperature. If room temperature is higher than 30ºC, shorten the deparaffinize time.
  3. The aim of differentiate is to increase the contrast. So that the differentiate time should be strictly controlled.
  4. For the smear sample or printed slice, start the H&E staining from step 5.
  5. Please use the product in the shelf life. In order to better preserve the product, heat, low temperature and light should be avoid.
  6. For your safety and health, please wear the lab coat and wear a disposable glove operation
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